Instruct-ERIC Events

EMBO Practical Course: High throughput Protein Production and Crystallization

Training
Date: 15-May-2013

Instruct

Structural proteomic projects have been established world-wide to carry out high throughput structure determination on a genome-wide scale. Crucial to this effort has been the development of HTP technologies for protein production, protein crystallization and improved methods for structure solution by X-ray crystallography. The overall aim of the course will be to review the state-of-the-art in HTP structural biology with an emphasis on methods to study complex targets, including membrane proteins.

Practical training will be provided in:

  • Structural bioinformatics (lecture & workshop)
  • High throughput cloning & expression in E. coli and mammalian cells (lectures & practicals)
  • Protein purification and biophysical characterisation (lectures, practicals and demonstrations).
  • High throughput protein crystallization (lecture & practicals).
  • High throughput data collection at synchrotrons (lectures & workshop).


Participants will be able to apply the protein production and crystallization methods to their own protein target(s) with the option of entering their target(s) into the process at various stages.

For more information, please visit:

http://events.embo.org/13-htp-protein/index.html