RNAsin expression / purification protocol

Dear Colleagues, I do have a clone for E.coli MBP- tagged RNAsin, but no information at all.

Has anyone experience with expression /purification?  I can also share the clone if someone would like to have it.

Best wishes

Posted on 17-Nov-2011 14:48 CET
Sabine Suppmann

Hi Sabine,

I have uploaded our protocol for MBP-RNAsin in the Files section. Just want to test if the exchange of protocols works. Would be easier to send by email of course, but who knows who else would need it.

Best regards


Posted on 22-Nov-2011 10:05 CET
Hüseyin Besir

Hi Hüseyin,

bingo!! Works nicely. Thank you again! Of course I also could have called you, but we can chat next week anyway......

Posted on 22-Nov-2011 15:44 CET
Sabine Suppmann

Hello Dr Hüseyin Besir,

Thank you for publishing the RNasin purification protocol. I wonder if you can provide us with a gene and expression protocol.

Best regards,

Vitaly Shevchenko

Forschungszentrum Juelich, ICS-5

Posted on 28-Feb-2012 14:22 CET
Vitaly Shevchenko
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