Secreted protein in insect cells

Dear All,

after having purified a secreted protein in insect cells, where its native SS was not correctly processed, we plan to change to that design: gp67 SS - StrepTag - Sumo Star Site - target protein.

Has anyone positive or negative experience with that setting?

Best

Sabine

 

Posted on 26-Nov-2012 15:59 CET

Hi Sabine

 

You probably know about this, but just in case, there is an on-line signal sequence predictor that could be of use here.

 

http://www.cbs.dtu.dk/services/SignalP/

 

Best

Bjørn

 

Posted on 27-Nov-2012 15:04 CET

Hi Sabine

 

You probably know about this, but just in case, there is an on-line signal sequence predictor that could be of use here.

 

http://www.cbs.dtu.dk/services/SignalP/

 

Best

Bjørn

 

Posted on 27-Nov-2012 15:04 CET
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