Z-basic tag for purification of secreted proteins

Dear all,

I would like to test a C-terminal Z-basic tag (according to Hedhammar and Hober, 2007) for the purification of proteins secreted from insect cells, and am wondering if somebody already tried if a fusion protein with the positively charged Z-basic domain got secreted well and could be captured by cation exchange chromatography from the culture medium?

With best regards,


Posted on 28-Oct-2013 18:26 CET
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