Thanks for your comment.
We routinely use HBM when expressing secretory proteins in insect cells. Your comments on better success rate, yields and quality with HEK is interesting. We haven't done a direct comparsion and would be great to see some of the data if you can share them.
I wish express a protein of bovine origin and that needs to secreted out into the medium when grow in BHK-21 cells/ in vivo. but the protein does not have any signal peptide..can anyone suggest how to proceed.