Does anyone have or know of a Mammalian vector with one constituve promoter and a second inducible promoter?
Failing this could anyone recommend a mammalian inducible promoter that I could use to put a vector together myself?
The best known and most commonly used inducible system is the Tet-On vectors, which you are most likely familiar with. I have however not heard about a vector as the one you describe.
I was wondering if you have thought about using an insect inducible system instead.
I have a modified version of the pMT-(BIP)-V5-His6 vector from Invitrogen where I added a Puromycin selection marker and a removable C-terminal HA/His10 tag.
Let me know if you are interested so I can send you more details.