VP-DSC calorimeter measures heat changes that occur in the sample (biomolecule solution) during a controlled increase or decrease in temperature, on the basis of a temperature difference between the sample and the reference material. It is a valuable technique for the study of samples in solution providing fast and accurate determination of the transition midpoint Tm - when 50 % of the biomolecule are unfolded. In a typical arrangement of ITC (Isothermal Titration Calorimetry), the titrant, also referred as the ligand, is injected into the sample cell containing the macromolecule sample solution. The calorimetric measurement can be done over a range of biologically relevant conditions (temperature, salt, pH, etc.).