The macromolecular crystallisation platform enables in-drop dynamic light scattering measurement to check the quality of the protein sample, robotic setup of 96-well crystallisation plates, incubation at selected temperature from a wide range, and automated monitoring of the crystallisation experiments. Experiments can be stored at 4-30 (or higher) deg Celsius. Dedicated rooms with stereomicroscopes for crystal manipulation are available at 20, 10 and 25 (or higher) deg Celsius. Inert atmosphere crystallisation is available. Equipment: Art Robbins Gryphon Dropsetter, DLS Spectrolight 600, Glovebox PETG10R320T3, Formulatrix RI 1000 Crystallisation Hotel, stereomicroscopes with imaging, cooled centrifuges, nanodrop spectrophotometer.
Crystallisation plate storage and automated crystallisation monitoring enclosure allowing remote access to crystallisation images and data evaluation. Crystallisation drop images are taken using visible light, polarised light and UV.
The glovebox equipped with a stereomicroscope allows users to manipulate proteins, usually for crystallisation experiments, under an inert atmosphere (usually nitrogen, other inert gases may be used on request).
A multi-channel (96 channels) pipetting robot for the easy set-up of nanodrop crystallisation plates. This crystallisation robot equipment allows the robotic setup of 96-well crystallisation plates, for the screening of crystallisation conditions (and also for “routine crystal production”) - for proteins, nucleic acids, complexes of biological macromolecules.
In drop dynamic light scattering measurements in Terasaki 72-well plates. This equipment allows checking the monodispersity of the macromolecule in drops, thereby allowing to check the suitability of the sample for crystallisation. It also allows time-lapse of crystallogenesis.
Preparation of biomolecule crystal samples for the measurement in the diffractometer. Handling of crystals, testing of cryo-conditions, cryo-cooling of crystalline samples.