Service / Technology Instance

About Crystallisation - Oxford

Access to Instruct crystallisation provides full use of the Strubi-crystallisation facility for setting up crystallisation experiments and monitoring these experiments via a web based GUI http://www.oppf.ox.ac.uk/xtalpims/. The PX-Scanner is an integral part of the automated crystallisation screening process, allowing fast in-situ analysis of crystals, which can significantly shorten the path to diffraction quality crystals.

View All Crystallisation OLD at Instruct

Instruments Available:

Access to Instruct crystallisation provides full use of the Strubi-crystallisation facility for setting up crystallisation experiments and monitoring these experiments via a web based GUI http://www.oppf.ox.ac.uk/xtalpims/. The PX-Scanner is an integral part of the automated crystallisation screening process, allowing fast in-situ analysis of crystals, which can significantly shorten the path to diffraction quality crystals.

User Guide

Typical sample volumes required are 15 ┬Ál per nanodrop crystallisation plate. The optimal protein concentration is determined with a pre-crystallisation test and is usually between 5 and 10 ml/mg. A wide range of primary screens and optimisation methods are available. Plates are set up at room temperature and then transferred to storage and imaging systems at 20.5 or 6.5 degrees. A general description of the facility can be found at Acta Cryst. D61, 651-657 (2005).

Instruct Centre

STRUBI

STRUBI

University of Oxford

WTCHG, Roosevelt Drive

OX3 7LF, Oxford

United Kingdom

www.strubi.ox.ac.uk

Contacts:

Yuguang Zhao
Yuguang Zhao
University of Oxford
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Karl Harlos
Karl Harlos
University of Oxford
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