Expression in mammalian cells at IGBMC Strasbourg

IGBMC-CERBM Strasbourg provides expression in mammalian cells.Over expression of proteins in mammalian cells (hamster BHK21 cells) is achieved using an attenuated vaccinia virus vector (Modified Vaccinia Virus Ankara-MVA) that is user safe and may be handled under BSL1 conditions. Genes of interest are cloned downstream of a bacteriophage T7 promoter in a first step to check for feasibility. Protein expression is then examined after plasmid transfection into BHK21 cells and co-infection with a specialized viral vector encoding the bacteriophage T7 RNA polymerase.

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