High Throughput Membrane Protein Crystallisation in Grenoble, France

The platform is fully equipped to accomplish successful in meso crystallization (lipidic cubic phase, LCP) of membrane proteins. Robotic nanovolume dispensers (NT8 and Mosquito) screen 96 precipitant conditions with small amount of sample (5µl per plate) in few minutes. Our platform works with commercially available kits specialized in membrane protein crystallization. Automated imaging systems, RockImager 1500 and 1000 (Formulatrix), visualize crystallization drops with visible and UV lights. HTMPC is now part of the HTX Lab (EMBL) and benefits from CrystalDirect technology developed at the HTX Lab. CrystalDirect plate allows automated crystal harvesting and is well adapted to crystals grown in LCP drops. LCP-FRAP (Formulatrix) which measures protein diffusion in lipid bilayer is also available and stands for pre-screening of crystallisation conditions.

View All Crystallisation at Instruct

 

Instruct Centre

Service/Technology Contacts

  • Florine Dupeux login to contact

User Guide

Key words

Structural Biology, high-throughput crystallisation, in meso, LCP, crystallisation, membrane protein, lipidic cubic phase, LCP-FRAP

Contact

htx@embl.fr

Dedicated staff

Dupeux Florine

Specific equipment

Robots for in meso, LCP membrane protein crystallization (nanodispenser), automated system for imaging crystallization plates, UV microscope for visualisation of protein crystals, temperature controlled rooms for crystallisation (20°C, 4°C), CRIMS to visualize images through internet, LCP-FRAP for protein mobility.

Access mode

Open access to internal and external users.

https://htxlab.embl.fr/

Cost

on demand

Location

CIBB/Platform  S05

CIBB/Office  120

How to make a request?

Register and send request forms on our https://htxlab.embl.fr/