Protein expression in mammalian cells - NKI

Depending on the project, the platform for expression will be chosen. Proteins can be expressed in E. coli, Insect cells and mammalian cells. For each of the systems we can do small scale (few ml) as well as large scale (few liters) production. Our mammalian expression system has currently been optimized for HEK-293T cells and is typically used for expression of extracellular (secreted) proteins. These cells can be cultured in flasks, dishes or rollerbottles. A glycosylation deficient strain - gnT1-deficient HEK293S - is also available for production of eukaryotic proteins that would benefit from removal of glycosyl chains (e.g. for crystallisation purposes). The use of other cells for expression can be discussed.

View All Mammalian Expression at Instruct


Instruct Centre

Service/Technology Contacts

  • Anastassis Perrakis login to contact
  • Reinout Raijmakers login to contact
  • Patrick Celie login to contact

User Guide

Please see the NKI protein facility website for more information.