Podcast Series: 2nd Best Practice in CryoEM Workshop
In October 2018, NeCEN hosted the Instruct 2nd Best Practice in CryoEM Workshop. Supported by Instruct-ULTRA, the aim was to share best practices between cryoEM centres for the delivery of services to academia and companies. Focus was given towards operations, remote control, efficient usage of the machines and live processing in two separate sessions followed by round table discussions. Day one began with four speakers presenting their facilities and how they operate (eBIC, EMBL Heidelberg, Leeds Astbury, Stockholm SciLifeLab). Plenaries followed with presentations on the status of cryoEM in Brazil and at the ESFR in Grenoble, France. Presentations on the second day looked at how four centres approach the issue of image processing, including live processing (IGBMC Strasbourg, CNB Madrid, Tsinghua University China, MPI Gottingen Germany).
An update on eBIC
Length: 28 minutes Presenter: Dr Daniel Clare, Instruct-UK
Dr Daniel Clare from University of Oxford gives an update on the CryoEM facilities at eBIC.
CryoEM at Leeds
Length: 35 minutes Presenter: Dr Rebecca Thompson
Dr Rebecca Thompson from Astbury University introduces the CryoEM facility at Leeds.
Unified EM setup: the benefits of SerialEM for facilities
Length: 30 minutes Presenter: Dr Wim Hagan, from EMBL Heidelberg
Dr Wim Hagen from EMBL Heidelberg describes the benefits of serialEM for research facilities.
Current status and perspectives of cryo-EM in Brazil
Length: 37 minutes Presenter: Dr Rodrigo Portugal
Dr Rodrigo Portugal discusses the current status and perspectives of Cryo-EM in Brazil.
Cryo-EM at the European Synchrotron
Length: 17 minutes Presenter: Dr Cristoph Mueller-Dickmann, from the European Synchrotron Radiation Facility
Dr Cristoph Mueller-Dickmann describes the Cryo-EM at the European synchrotron.
Pipeline data collection at the IGBMC cryo-EM platform
Length: 24 minutes Presenter: Dr Ottilie von Loeffelholz
Dr Ottilie von Loeffelholz gives a talk on the collection of pipeline data at the IGMBC Cryo-EM Platform.
Scipion for facilities
Length: 40 minutes Presenter: Dr Pablo Conesa
Dr Pablo Conesa discusses the use of scipions for facilities
System for high-throughput cryo-EM at atomic resolution
Length: 35 minutes Presenter: Dr Xueming Li, from the University of Tsinghua
Dr Xueming Li from University of Tsinghua gives a talk on the systems for high-throughout Cryo-EM at atomic resolution
Cryo-EM data pre-processing at full Warp
Length: 28 minutes Presenter: Dr Dmitry Tegunov
Dr Dmitry Tegunov discusses Cryo-EM data pre-processign at full warp
Swedish national Cryo-EM facility
Length: 34 minutes Presenter: Dr Marta Carroni, from the University of Stockholm
Dr Marta Carroni describes the current Swedish national Cryo-Em facility in Stockholm