Techniques are provided for the biophysical characterization of the structure, function and stability of biological macromolecules like proteins, nucleic acids, lipids and their complexes (Circular Dichroism, Differential Scanning Fluorescence, Differential Scanning Calorimetry, Dynamic Light Scattering, UV/Vis Spectrometer) and kinetic and thermodynamic parameters of their iteration (Microscale Thermophoresis, Surface Plasmon Resonance, Isothermal titration Calorimetry).
The macromolecular crystallisation platform enables in-drop dynamic light scattering measurement to check the quality of the protein sample, robotic setup of 96-well crystallisation plates, incubation at selected temperature from a wide range, and automated monitoring of the crystallisation experiments. Experiments can be stored at 4-30 (or higher) deg Celsius. Dedicated rooms with stereomicroscopes for crystal manipulation are available at 20, 10 and 25 (or higher) deg Celsius. Inert atmosphere crystallisation is available. Equipment: Art Robbins Gryphon Dropsetter, DLS Spectrolight 600, Glovebox PETG10R320T3, Formulatrix RI 1000 Crystallisation Hotel, stereomicroscopes with imaging, cooled centrifuges, nanodrop spectrophotometer.
D8 Venture diffractometer (Bruker) with a MetalJet D2 high-flux liquid Gallium X-ray source, Photon II detector and Kappa goniometer. This diffractometer is used for X-ray diffraction studies of biomolecular crystals (macromolecular crystallography). The device is also equipped with an ISX motorised stage for in-situ X-ray diffraction experiments, enabling screening of diffraction properties in crystallisation plates (plus limited diffraction data collection). The beam size of 70 microns (FWHM) and its high brilliance enable very effective screening, automated in-plate screening and fast data collection. Typical exposure time per degree for protein crystals: 30-90 seconds.
Structural mass spectrometry (MS3D) offers various methodologies for characterization of protein structure. At BIOCEV we offer different labelling approaches including hydrogen/deuterium exchange, covalent labelling, chemical cross-linking and limited proteolysis. We are equipped with cutting-edge technologies including high-resolution mass spectrometer, HPLC system, H/D system and in-house software for data processing. The service we provide includes data processing and reporting ready for publication. Besides the MS3D we offer the following services: Identification and quantification of proteins. Precise determination of protein molecular mass. Characterization of various posttranslational modifications.
Visit this centre and more by submitting a proposal for access.Submit Proposal