Instruct Centres > Instruct Centre - FR1

Strasbourg provides an integrated environment for structural studies of protein and complexes from sample preparation to structure determination.

Instruct Centre - FR1

Flagship Service/Technology

Electron Microscopy, Strasbourg, France

The center offers access for three-dimensional structure determination using cryo-electron microscopy (cryo-EM).The centre has expertise in all aspects of single particle analysis from the automated acquisition of large data sets to image analysis . Access to the following instrumentation is provided: The Tecnai F30 Polara cryo electron microscope allows high-resolution SPA and CET automated data collection. The Titan Krios cryo electron microscope with a CMOS (FEI FALCON 4K*4K) high-sensitivity direct electron detector camera. The Tecnai F20 is equipped with a FEG operated at 100keV or 200keV.

View All *Electron Microscopy at Instruct

 

Instruct Centre - FR1


The Strasbourg center provides a cutting-edge technological environment for integrative structural biology approaches, from the molecular to the cellular level, with an emphasis on protein production linked with preparation of samples for EM and crystallography.


Protein Production

Protein engineering and production are essential tools for structural, biophysical and functional studies as well as for biotechnology and medical applications. The Strasbourg center offers a state-of-the-art infrastructure for the production of biomolecules and biomolecular complexes in E. coli, yeast, insect cells (baculovirus expression system) as well as in mammalian cells using an attenuated vaccinia virus vector. The Strasbourg platform has a unique expertise on production of recombinant multi-protein complexes as well as on purification of endogenous complexes using the TAP-tag technology in yeast.


Structure Determination

Strasbourg provides core-technologies for structure determination by crystallography, cryo-EM and tomography. Strasbourg is a center of expertise for crystrallographic analysis of protein-protein and protein-nucleic acid complexes (nano and micro-automated screening and HTP crystal production, data collection and structure determination) and cryo-electron microscopy/tomography (Hight resolution cryo-EM, cryo electron tomography using up to date equipment (Polara and Titan microscopes and Falcon detectors).


The center also offers access to:

  • biophysical platform to monitor protein properties and function, including protein stability, protein complex formation, oligomerisation and protein interactions,
  • shape determination by SAXS.


Visit This Centre

Welcome to IGBMC Srasbourg, France.


You will find information on "Travelling to IGBMC" at www.igbmc.fr/igbmc/plan/


To find advice on "Life in Strasbourg" you can download a pdf file: http://archive.igbmc.fr/igbmc/LifeInStrasbourg.pdf

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Scientific Contacts

  • Alberto Podjarny login to contact
  • Jean Cavarelli login to contact

Admin Contacts

  • Marie-Christine Poterszman login to contact

Technical Contacts

  • Catherine Birck login to contact