Biophysical Characterisation of Macromolecules and Quantification of Biomolecular Interactions

Training
Date: 20-Nov-2017 0:00 CET to 23-Nov-2017 16:00 CET

Contact: Anastassis Perrakis

Instruct

What is this course about? The course aims to provide the theoretical background of popular biophysical methods that are often used in relation to Structural Biology research, and practical experience both in performing experiments and in analysing the resulting data.

 


Why should I attend? The rationale behind an extended kit of experiments to characterise macromolecules and their interactions will be explained. You will be shown how to perform and analyse the related experiments in dedicated practical sessions.

 

The course is limited to 12 spaces. Therefore, please do not make any travel or other arrangements before being notified of your application outcome.

 

You can apply online for the event via this link.

List of Lectures

  • Methods in Structural Audit, Patrick Celie
  • Principles of ITC and kinetic applications, Philippe Dumas
  • Surface Plasmon Resonance, Alex Fish
  • Basics of fluorescence methods, Anastassis Perrakis & Alex Fish
  • The Chemical Realm of Nature and the Nature of Chemistry, Nikos Psarros
  • Haddock: modelling macromolecular interactions, Alexandre Bonvin
  • Microscale Thermophoresis, Katarzyna Walkiewicz
  • Understanding enzymes by kinetics analysis, Kenneth Johnson
  • Advanced application of fluorescence methods, Peter Friedhoff
  • Global Fitting of kinetic data, Kenneth Johnson

 




List of Practicals

  • Structural Audit Procedure
  • Surface Plasmon Resonance
  • Isothermal Titration Calorimetry
  • Miscroscale Thermophoresis 
  • Fluoresence Methods 
  • Stopped Flow 
  • Graphpad/Prism 
  • Kintek 

Organisers

  • Patrick Celie
  • Alexander Fish
  • Tatjana Heidebrecht
  • Anastassis Perrakis
  • Titia K. Sixma



Detailed Programme



Monday November 20


9:00 - 9:15

Welcome and organisational issues, Anastassis Perrakis

9:15 - 10:00

Methods in Structural Audit, Patrick Celie

 

Cofee

10:30 - 11:15

Principles of ITC and kinetic applications, Philippe Dumas

11:15 - 12:00

Surface Plasmon Resonance, Alex Fish

 

Lunch

13:00 - 15:30

Practicals P1 / P2 / P3

 

Coffee

16:00 - 18:30

Practicals P2 / P3 / P1

 

Dinner at "Radeon" near NKI

 

Tuesday November 21

 

9:00 - 10:00

Basics of fluorescence methods, Anastassis Perrakis & Alex Fish

 

Coffee

10:30 - 11:15

Haddock: modelling macromolecular interactions, Alexandre Bonvin

11:15 - 12:00

Microscale Thermophoresis, Katarzyna Walkiewicz

 

Lunch

13:00 - 15:30

Practicals P3 / P2 / P1

 

Coffee

16:00 - 18:30

Practicals P4 / P5 / P6

 

Free evening

 

Wednesday November 22

 

9:00 - 10:00

Understanding enzymes by kinetics analysis, Kenneth Johnson

 

Coffee

10:30 - 13:00

Practicals P5 / P6 / P4

 

Lunch

13:45 - 14:30

Advanced application of fluorescence methods, Peter Friedhoff

14:30 - 17:00

Practicals P6 / P4 / P5

 

Coffee

17:30 - 19:00

Open Discussion on Experimental Strategy and Planning

 

Pizza Dinner at NKI

20:00 - 21:00

The Chemical Realm of Nature and the Nature of Chemistry, Nikos Psaros

 

Thursday November 23

 

9:00 - 11:30

Practical P7 (computational)

11:30 - 13:00

Global Fitting of kinetic data, Kenneth Johnson

 

Lunch

13:30 - 16:00

Practical P8 (computational)

Departure

 

 

 

NKI, Amsterdam, Netherlands

Biophysical Characterisation of Macromolecules and Quantification of Biomolecular Interactions