Biophysical Characterisation of Macromolecules and Quantification of Biomolecular Interactions

Training

Date: 20-Nov-2017 to 23-Nov-2017

Contact: Anastassis Perrakis

Instruct

What is this course about? The course aims to provide the theoretical background of popular biophysical methods that are often used in relation to Structural Biology research, and practical experience both in performing experiments and in analysing the resulting data.

Why should I attend? The rationale behind an extended kit of experiments to characterise macromolecules and their interactions will be explained. You will be shown how to perform and analyse the related experiments in dedicated practical sessions.

The course is limited to 12 spaces. Therefore, please do not make any travel or other arrangements before being notified of your application outcome.

You can apply online for the event via this link.

List of Lectures

Methods in Structural Audit, Patrick Celie
Principles of ITC and kinetic applications, Philippe Dumas
Surface Plasmon Resonance, Alex Fish
Basics of fluorescence methods, Anastassis Perrakis & Alex Fish
The Chemical Realm of Nature and the Nature of Chemistry, Nikos Psarros
Haddock: modelling macromolecular interactions, Alexandre Bonvin
Microscale Thermophoresis, Katarzyna Walkiewicz
Understanding enzymes by kinetics analysis, Kenneth Johnson
Advanced application of fluorescence methods, Peter Friedhoff
Global Fitting of kinetic data, Kenneth Johnson

List of Practicals

Structural Audit Procedure
Surface Plasmon Resonance
Isothermal Titration Calorimetry
Miscroscale Thermophoresis
Fluoresence Methods
Stopped Flow
Graphpad/Prism
Kintek

Organisers

Patrick Celie
Alexander Fish
Tatjana Heidebrecht
Anastassis Perrakis
Titia K. Sixma

Detailed Programme

Monday November 20

9:00 - 9:15	Welcome and organisational issues, Anastassis Perrakis
9:15 - 10:00	Methods in Structural Audit, Patrick Celie
	Cofee
10:30 - 11:15	Principles of ITC and kinetic applications, Philippe Dumas
11:15 - 12:00	Surface Plasmon Resonance, Alex Fish
	Lunch
13:00 - 15:30	Practicals P1 / P2 / P3
	Coffee
16:00 - 18:30	Practicals P2 / P3 / P1
	Dinner at "Radeon" near NKI

Tuesday November 21

9:00 - 10:00	Basics of fluorescence methods, Anastassis Perrakis & Alex Fish
	Coffee
10:30 - 11:15	Haddock: modelling macromolecular interactions, Alexandre Bonvin
11:15 - 12:00	Microscale Thermophoresis, Katarzyna Walkiewicz
	Lunch
13:00 - 15:30	Practicals P3 / P2 / P1
	Coffee
16:00 - 18:30	Practicals P4 / P5 / P6
	Free evening

Wednesday November 22

9:00 - 10:00	Understanding enzymes by kinetics analysis, Kenneth Johnson
	Coffee
10:30 - 13:00	Practicals P5 / P6 / P4
	Lunch
13:45 - 14:30	Advanced application of fluorescence methods, Peter Friedhoff
14:30 - 17:00	Practicals P6 / P4 / P5
	Coffee
17:30 - 19:00	Open Discussion on Experimental Strategy and Planning
	Pizza Dinner at NKI
20:00 - 21:00	The Chemical Realm of Nature and the Nature of Chemistry, Nikos Psaros

Thursday November 23

9:00 - 11:30	Practical P7 (computational)
11:30 - 13:00	Global Fitting of kinetic data, Kenneth Johnson
	Lunch
13:30 - 16:00	Practical P8 (computational)
Departure

NKI, Amsterdam, Netherlands

Biophysical Characterisation of Macromolecules and Quantification of Biomolecular Interactions

Instruct-ERIC Events