Instruct-ERIC Events

Instruct training course on fully remote data collection on eBIC CryoEMS

Training
Date: 10-May-2021

Contact: Alistair Siebert

Instruct

At eBIC a course on fully remote data collection will take place on 10th May 2021 funded by Instruct-ERIC.

Outlined course content

  • The course will begin by introducing our preferred tools for discussing planned experiments with users.
  • An introduction to IspyB mediated sample shipping for cryo-EM will follow.
  • Users will be led through our NoMachine mediated FEDID keyed login to the microscopes. 
  • A discussion of EPU concepts and imaging mode pre-sets will be followed by demonstrations of grid screening best practice with automated tools. 
  • Instructors will then demonstrate best practices in data acquisition methodologies on our BioQuantum K3 equipped Titan Krios. 
  • Finally, the IspyB mediated on-the-fly data processing pipeline setup for monitoring and assessing data collection will be demonstrated.
  • The course will provide 2x 8hr Talos sessions & 1 x 8 hr Krios session. Krios sessions can be extended for data collection purposes on user's samples to 48 hrs but this will require prior evidence of high-quality samples and high-resolution 2d classes and a science case justification for time.

 

More information to follow soon

 

Harwell, United Kingdom

Instruct training course on fully remote data collection on eBIC CryoEMS