Instruct-ERIC Events

NCCAT Tomography Shortcourse

Registration Date: 26-Nov-2019 to 01-Dec-2019
Date: 13-Apr-2020 to 18-Apr-2020

SEMC and NCCAT will be offering a 1 week workshop focused on the theory and practice of tomographic methods. The mornings will have lectures and stimulating round table discussions. The afternoons provide hands-on practicals to reinforce fundamental concepts and topics covered earlier in the day.

Day 1: Monday, April 13, 2020
Roundtable: Welcome
Lecture 1: Keynote – Intro and overview of ET – Wei Dai (Rutgers University)
Practical 1: ET sample preparation – HPF/Plunge freezers
Practical 2: cryoCLEM-FIB-ET workflow

Day 2: Tuesday, April 14, 2020
Lecture 2: FIB-SEM – Bill Rice (New York University)
Roundtable: Making windows into cells
Practical 3: Krios cryoET data collection

Day 3: Wednesday, April 15, 2020
Lecture 3: Cryo-Applications and sub-tomogram averaging – Alex Noble (NYSBC/SEMC)
Roundtable: Choosing the right in situ target and model system
Practical 4: Appion Protomo tools and sub-tomogram averaging

Day 4: Thursday, April 16, 2020
Lecture 4: Annotation and interpretation of tomographic data – TBD
Roundtable: The future of in situ cellular structural biology
Practical 5: Appion Protomo tools and sub-tomogram averaging (cont.)
Practical 6: Annotation using CNN
Group dinner

Day 5: Friday, April 17, 2020
Roundtable: Wrap up and Farewell

For further information or to apply for a place, click here.

NCCAT, 89 Convent Avenue, New York, United States

NCCAT Tomography Shortcourse