The research groups of Prof. Amedeo Caflisch (Department of Biochemistry) and Prof. Cristina Nevado (Department of Chemistry) at the University of Zurich are looking for a post-doctoral associate with strong experience in cryo-electron microscopy and single-particle analysis. The researcher is expected to contribute expertise in single-particle cryo-EM to support ongoing collaborative projects aiming at the optimization and characterization of in-house designed proteolysis targeting chimeric (PROTAC) molecules that show nanomolar affinity on cancer-relevant targets. On a second stage, the postdoctoral researcher will have the possibility to manage her/his own independent research project within the main research focus areas of the host laboratory. The researcher will have access to the Center for Microscopy and Image Analysis in the same building on campus.
Your responsibilities
The activities of this position involve mainly:
Protein expression (mainly in E. coli) and purification
Cryo-EM sample handling and preparation protocols including negative staining and sample vitrification
Process single particle cryo-EM data including handling raw data, interpretation, 3D reconstruction and map refinement, and model building
Structure-based optimization of small-molecule ligands and PROTACs
Your profile
Experience on data-processing and structure solution of small size protein complexes (<150 kDa) is desired. The candidate should be about to receive their PhD degree or have less than three years of previous post-doctoral experience. Applications from senior researchers will not be considered.
What they offer
Our well-funded drug discovery research program combines inspiring international research environment with access to state-of-the-art scientific infrastructure. Our alumni value their comprehensive training: about 25% of them are offered a tenure track assistant professorship position, with the vast majority of the rest finding employment in large pharma or biotech industry.
Place of work
Irchel campus, CH-8057 Zurich, Switzerland
Start of employment
Starting date: by arrangement (immediate entry possible). Length: 3 years with an initial 1-year contract. Applications are to be submitted as a single PDF file to caflisch@bioc.uzh.ch and must contain a cover letter, a concise summary of past accomplishments in cryo-EM, a CV (of maximum two pages), and at least one letter of support or the names and contact details of two (former) advisors.
