SILAC labelled insect cell medium

Dear Colleagues,

has anyone grown / produced in insect cells in SILAC medium (Arg- Lys -)?

Thanks

Best

Sabine

Posted on 27-Jan-2016 12:49 CET

Hi Sabine,

this is described in the paper of Nuno Carinhas from the group of Paula Alves.

They use Sf900II without arg and without lys. The method is described in

doi:10.1371/journal.pone.0026444

We have no experience with the SILAC method in Insect cells but they can help you for sure.

Sincerely yours Joop van den Heuvel

 

Posted on 27-Jan-2016 13:48 CET