IGBMC Strasbourg bacterial expression platform combines (i) a large set of expression vectors for Escherichia coli and (ii) robotized protocols, which enable a broad screening of soluble protein expression through the use of multiple fusion proteins and various growth conditions.IGBMC Strasbourg bacterial platform has also a long standing and unique expertise in protein complexes reconstitution and production by multi-expression in Escherichia coli. This technology is fully compatible with the automated protocols used for single expression.All bacterial expression vectors provided by the platform are compatible with major cloning strategies (e.g. restriction, Gateway, SLIC).
IGBMC bacterial expression platform offers two modes of access:
1. The platform provides the expression vectors to the external users who perform cloning at their home institutions and come to IGBMC to perform the expression assays.
2. The external users perform cloning and expression assays at the IGBMC platform.
Cloning at home institution might be useful when large series of vectors have to be produced and to avoid unexpected delays which are sometimes inherent to the cloning activity. The user deciding to perform cloning in his/her own laboratory will receive, prior to cloning, counsel from the IGBMC platform to design the best expression strategy according to his/her project. Even if initial cloning is performed at the home institution, additional cloning at the IGBMC platform will be carried out if analysis of the initial expression tests suggests that this is required for the correct completion of the project.
Support at the IGBMC platform includes training, supervision, and, importantly, help with data analysis that is especially crucial for complex reconstitution. This help can also be provided once the applicant has returned to his/her home laboratory, thus extending the impact of the visit.