Access to Instruct crystallisation provides full use of the Strubi-crystallisation facility for setting up crystallisation experiments and monitoring these experiments via a web based GUI http://www.oppf.ox.ac.uk/xtalpims/. The PX-Scanner is an integral part of the automated crystallisation screening process, allowing fast in-situ analysis of crystals, which can significantly shorten the path to diffraction quality crystals.
Typical sample volumes required are 15 µl per nanodrop crystallisation plate. The optimal protein concentration is determined with a pre-crystallisation test and is usually between 5 and 10 ml/mg. A wide range of primary screens and optimisation methods are available. Plates are set up at room temperature and then transferred to storage and imaging systems at 20.5 or 6.5 degrees. A general description of the facility can be found at Acta Cryst. D61, 651-657 (2005).
Users will be registered at the platform through https://www.oppf.ox.ac.uk/User/register.jsf followed by, at a later stage, their samples and aliquots.
Images of crystallisation experiments can be accessed via xtalPIMS: https://www.oppf.ox.ac.uk/xtalpims/Home.jsp.