Crystallisation - Oxford

Access to Instruct crystallisation provides full use of the Strubi-crystallisation facility for setting up crystallisation experiments and monitoring these experiments via a web based GUI http://www.oppf.ox.ac.uk/xtalpims/. The PX-Scanner is an integral part of the automated crystallisation screening process, allowing fast in-situ analysis of crystals, which can significantly shorten the path to diffraction quality crystals.

Instruct platform information

 

Crystallisation - Oxford

Service/Technology Contacts

User Guide

Typical sample volumes required are 15 ┬Ál per nanodrop crystallisation plate. The optimal protein concentration is determined with a pre-crystallisation test and is usually between 5 and 10 ml/mg. A wide range of primary screens and optimisation methods are available. Plates are set up at room temperature and then transferred to storage and imaging systems at 20.5 or 6.5 degrees. A general description of the facility can be found at Acta Cryst. D61, 651-657 (2005).

Users will be registered at the platform through https://www.oppf.ox.ac.uk/User/register.jsf followed by, at a later stage, their samples and aliquots.

Images of crystallisation experiments can be accessed via xtalPIMS: https://www.oppf.ox.ac.uk/xtalpims/Home.jsp.