Service / Technology Instance

About Electron Microscopy, Strasbourg, France

The center offers access for three-dimensional structure determination using cryo-electron microscopy (cryo-EM). The centre has expertise in all aspects of single particle analysis from the automated acquisition of large data sets to image analysis. Access to the following instrumentation is provided: the Tecnai F30 Polara cryo-electron microscope which allows high-resolution SPA and CET automated data collection and the Titan Krios cryo-electron microscope with a CMOS (FEI FALCON 4K*4K) high-sensitivity direct electron detector camera. The Tecnai F20 is equipped with a FEG operated at 100keV or 200keV.

Instruments Available:

The center offers access for three-dimensional structure determination using cryo-electron microscopy (cryo-EM).The centre has expertise in all aspects of single particle analysis from the automated acquisition of large data sets to image analysis . Access to the following instrumentation is provided: The Tecnai F30 Polara cryo electron microscope allows high-resolution SPA and CET automated data collection.

The center offers access for three-dimensional structure determination using cryo-electron microscopy (cryo-EM).The centre has expertise in all aspects of single particle analysis from the automated acquisition of large data sets to image analysis . Access to the following instrumentation is provided: The Tecnai F20 is equipped with a FEG operated at 100keV or 200keV.

The center offers access for three-dimensional structure determination using cryo-electron microscopy (cryo-EM). The centre has expertise in all aspects of single particle analysis from the automated acquisition of large data sets to image analysis . The Titan Krios cryo electron microscope is the latest generation electron microscope with capacities for high-resolution data collection for both Cryo Electron Tomography (CET) experiments and Single Particle Analysis (SPA) with automated data collection. The electron source is a Field Emission Gun (FEG) that can be operated at 80keV, 100keV, 200keV or 300keV. An automatic loading mechanism allows mounting twelve grids at a time. This microscope is equipped with CMOS (FEI FALCON3 4K*4K and GATAN K2) high-sensitivity direct electron detector cameras, Cs corrector, phase plate and GIF.

User Guide

The Titan Krios cryo electron microscope

The Titan Krios the latest generation electron microscope with capacities for high-resolution data collection for both Cryo Electron Tomography (CET) experiments and Single Particle Analysis (SPA) with automated data collection. The electron source is a Field Emission Gun (FEG) that can be operated at 80keV, 100keV, 200keV or 300keV. An automatic loading mechanism allows mounting twelve grids at a time. This microscope is equipped with CMOS (FEI FALCON3 4K*4K and GATAN K2) high-sensitivity direct electron detector cameras, corrector CS, phase plate and GIF.

The Tecnai F30 Polara

The Tecnai F30 Polara cryo-electron microscope allows high-resolution SPA and CET automated data collection. Its FEG is usually tuned to 100keV, 200keV or 300keV. The grids are manually mounted six at a time. This microscope is equipped with three digital cameras: FEI CMOS 4K*4K "FALCON", FEI CCD 4K*4K "EAGLE" and a GATAN CCD 2K*2K "ORIUS".

The Tecnai F20

The Tecnai F20 is equipped with a FEG operated at 100keV or 200keV. Grids are mounted one at a time using a Gatan side-entry cryo-holder. This microscope is equipped with one digital camera (GATAN CCD 2K*2K "US10001"), it is used to collect data for cryo-SPA or room temperature electron tomography using a Fischion side-entry holder. Cryo-EM grids are flash-frozen in a temperature and humidity controlled environment using a Vitrobot system (FEI).

All the microscopes are equipped with digital cameras, images can be recorded as films and scan them with a high-resolution Heidelberger Druckmaschinen drum scanner (5 micron pixel size)When asking access for Titan or Polara, please add to your proposal preliminary image of the sample in cryo condition.

Instruct Centre

Instruct Centre FR1

IGBMC

1, rue Laurent Fries

Illkirch

Strasbourg

France

www.igbmc.fr

Electron Microscopy, Strasbourg, France

Contacts:

Marie-Christine Poterszman
Marie-Christine Poterszman
CNRS
Login to contact
Corinne Crucifix
Corinne Crucifix
IGBMC-CERBM
Login to contact
Tat Cheung Cheng
Tat Cheung Cheng
IGBMC-CERBM
Login to contact
Alexandre Durand
Alexandre Durand
Inserm
Login to contact