EM CEITEC - Instruct-CZ

Key equipment: High-end electron microscope for high-resolution cryoEM and cryo-tomography FEI Titan Krios (80 - 300 kV) equipped with an energy filter and a direct detector camera. Conventional cryoEM microscope FEI F20 (200 kV) equipped with a CCD camera and a dual beam FIB/SEM instrument (Versa3D) for micromachining of thin lamellas of vitrified cells usable for electron tomography.

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Instruct Centre

Service/Technology Contacts

  • Jiri Novacek login to contact
  • Katerina Vagnerova login to contact
  • Veronika Mikitova login to contact
  • Veronika Papouskova login to contact

User Guide

Instruments available:

  • FEI Titan Krios – the microscope operates at 300 kV and is equipped with an autoloader, FEI Falcon II direct detector, Gatan Quantum 964 energy filter and a post-GIF 4k CCD camera. The microscope is optimally suitable for high-resolution single particle cryo-electron microscopy and cellular cryo-electron tomography.
  • FEI Tecnai F20 – the microscope operates at 120–200 kV and is equipped with an FEI Eagle 4k CCD camera, two side-entry cryo-holders (Gatan 626 and Gatan 914) and two room-temperature side-entry holders. The microscope is optimally equipped for optimization of cryoEM samples and automated collection of data for initial 3-D reconstructions by single particle analysis.
  • FEI Versa3D – this small dual beam microscope (SEM/FIB) is equipped with a Quorum cryo-stage for insertion and processing of cryo-TEM grids (imaging by SEM and lamellae milling with FIB). The samples can be Pt-coated inside the microscope.

Services provided:

The cryo-EM core facility provides access and support to collect cryo-electron microscopy images for both single particle as well as for electron tomography applications. Furthermore, the facility provides support for sample preparation, namely plunge freezing of purified protein complexes as well as cryo-FIB lamella milling for thick biological objects such as cells. Additional support is given in experiment design, setting up data collection and data analysis (i.e. image processing). Interested non-specialists could receive training in using the electron microscopes or develop a collaboration with the CryoEM research group, particularly in these areas:

  • high-resolution structure determination of macromolecular complexes and assemblies
  • structural studies of intracellular compartments and host-pathogen interactions
  • time-resolved electron microscopy of transient macromolecular complexes