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About Macromolecular Crystallisation, Vestec near Prague, Czech Republic

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Instruments Available:

Crystallisation plate storage and automated crystallisation monitoring enclosure allowing remote access to crystallisation images and data evaluation. Crystallisation drop images are taken using visible light, polarised light and UV.

Nanoliter-volume crystallisation robot with drop dispensing capability, including setting drops for Lipidic Cubic Phase (LCP) experiments. The 8-tip head aspirates and dispenses drops from 10 nL to 1.5 μL. The instrument is able to set up hanging drop, sitting drop, microbatch, in meso LCP drops, additive and seeding experiments. Proportionally-Controlled Active Humidification prevents sample evaporation and increases experiment reproducibility.

The glovebox equipped with a stereomicroscope allows users to manipulate proteins, usually for crystallisation experiments, under an inert atmosphere (usually nitrogen, other inert gases may be used on request).

A multi-channel (96 channels) pipetting robot for the easy set-up of nanodrop crystallisation plates. This crystallisation robot equipment allows the robotic setup of 96-well crystallisation plates, for the screening of crystallisation conditions (and also for “routine crystal production”) - for proteins, nucleic acids, complexes of biological macromolecules.

In drop dynamic light scattering measurements in Terasaki 72-well plates. This equipment allows checking the monodispersity of the macromolecule in drops, thereby allowing to check the suitability of the sample for crystallisation. It also allows time-lapse of crystallogenesis.

Preparation of biomolecule crystal samples for the measurement in the diffractometer. Handling of crystals, testing of cryo-conditions, cryo-cooling of crystalline samples.

User Guide

The macromolecular crystallisation platform enables in-drop dynamic light scattering measurement to check the quality of the protein sample, robotic setup of 96-well crystallisation plates, incubation at selected temperature from a wide range, and automated monitoring of the crystallisation experiments. Experiments can be stored at 4-30°C (or higher). Dedicated rooms with stereomicroscopes for crystal manipulation are available at 20°C, 10°C and 25°C (or higher). Inert atmosphere crystallisation is available. Equipment: Formulatrix NT8 Dropsetter, Art Robbins Gryphon Dropsetter, DLS Spectrolight 600, Glovebox PETG10R320T3, Formulatrix RI 1000 Crystallisation Hotel, stereomicroscopes with imaging, cooled centrifuges, nanodrop spectrophotometer.

Instruct Centre

BIOCEV

BIOCEV

Prumyslova 595

25250, Vestec, Prague

Czech Republic

www.biocev.eu

Macromolecular Crystallisation, Vestec near Prague, Czech Republic

Contacts:

Jan Stránský
Jan Stránský
Institute of Biotechnology ASCR
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Jiri Pavlicek
Jiri Pavlicek
BIOCEV - Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University in Vestec
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