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About X-ray Diffraction and Bio-SAXS, Vestec near Prague, Czech Republic

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Instruments Available:

D8 Venture diffractometer (Bruker) with a MetalJet D2 high-flux liquid Gallium X-ray source, Photon II detector and Kappa goniometer. This diffractometer is used for X-ray diffraction studies of biomolecular crystals (macromolecular crystallography). The device is also equipped with an ISX motorised stage for in-situ X-ray diffraction experiments, enabling screening of diffraction properties in crystallisation plates (plus limited diffraction data collection).

Processing of X-ray diffraction data. Upon request, assistance with structure determination is also offered.

Preparation of biomolecule crystal samples for the measurement in the diffractometer. Handling of crystals, testing of cryo-conditions, cryo-cooling of crystalline samples.

The versatile SAXSpoint 2.0 (Anton Paar) SAXS/WAXS device is equipped with the latest high brilliance C2+ liquid MetalJet source (liquid gallium alloy anode) and a Dectris EIGER 1M detector. Liquid samples are loaded automatically using the automated high performance sampler or manually in flow through measurement cells, including a high S/N silicon nitride measurement cell. Measurements are performed in the temperature range -10 °C to 120 °C. The state of the sample can be monitored online using in situ UV-Vis spectroscopy to identify radiation damage, and measure precise sample concentration and aggregation state.

User Guide

X-ray difraction with possible in situ

D8 Venture diffractometer (Bruker) with a MetalJet D2 high-flux liquid Gallium X-ray source, Photon II detector and Kappa goniometer. This diffractometer is used for X-ray diffraction studies of biomolecular crystals. The device is also equipped with an ISX motorised stage for in-situ X-ray diffraction experiments, enabling screening of diffraction properties in crystallisation plates, plus limited diffraction data collection. The beam size of 70 microns (FWHM) and its high brilliance enable very effective screening, automated in-plate screening and fast data collection. Typical exposure time per degree for protein crystals: 30-90 seconds.

Small angle X-ray scattering

Versatile instrument to perform SAXS or WAXS experiments on liquid samples, in the temperature range -10° C to 120° C. SAXSpoint 2.0 (Anton Paar) is equipped with the latest high brilliance source MetalJet C2+ having a liquid gallium alloy anode, and a Dectris EIGER 1M detector. Samples can be loaded using the automated high precision sampler or manually in capillaries, including a high S/N silicon nitride measurement cell. The state of the sample can be monitored online using UV-Vis spectroscopy to identify radiation damage, measure precise sample concentration and aggregation state.

Instruct Centre

BIOCEV

BIOCEV

Prumyslova 595

25250, Vestec, Prague

Czech Republic

www.biocev.eu

X-ray Diffraction and Bio-SAXS, Vestec near Prague, Czech Republic

Contacts:

Jan Stránský
Jan Stránský
Institute of Biotechnology ASCR
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Jiri Pavlicek
Jiri Pavlicek
BIOCEV - Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University in Vestec
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