AFM - Instruct-CZ

Expertise: • Studies of biomolecules and other bio-objects at the molecular level using scanning probe techniques, i.e. atomic force microscopy (AFM) and related techniques (STM, SNOM, electrochemical variants), including combinations with optical microscopy (inverted, fluorescence, confocal) • Production and bioconjugation of nanoparticles • Advanced methods - nanolithograpy, nanomechanical manipulations, ink-jet based deposition

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Instruct Centre

Service/Technology Contacts

  • Jan Pribyl login to contact
  • Katerina Vagnerova login to contact

User Guide

Available instruments and techniques:

  • scanning probe microscope - Ntegra Vita / Solaris (NTMDT) - exchangeable measuring heads for AFM, STM, SNOM, electrochemical AFM, nanolithography
  • atomic force microscope NanoWizzard3 (JPK) mounted on the inverted confocal fluorescence microscope IX81 CLSM FV1200 (Olympus)
  • ForceRobot 300 (JPK) - automated force-distance curves for molecular nano-biointeractions
  • automated system SolverNEXT (NTMDT) - basic AFM scanning
  • fast-scanning AFM for biointeraction studies FastScanBio (Bruker)
  • ink-jet based deposition system SciFlex Arrayer S3 (Scienion) - preparation of microarrays

Services provided:

The imaging of biomolecules, cells and other biological structures and objects is realized in aqueous solution or in the dry state. The carrier materials for sample deposition range from ultra flat mica slides suitable for atomic resolution to highly oriented graphite, gold, silicon, glass and polymers as polystyrene petri dishes. Different scanning tips with appropriate sharpness and cantilevers with a wide range of force constants.

  • preparation of samples for AFM – fixation at the support materials
  • visualization and studies of samples using atomic force microscopy (AFM) both in dry state and in liquid, imaging using contact mode (static) and dynamic modes as tapping (intermittent contact, amplitude modulation AFM) and non-contact (frequency modulation AFM)
  • AFM combined with other techniques: electrochemistry, optical and confocal fluorescence microscopy
  • force spectroscopy - automated generation and evaluation of force-distance curves for tips and surfaces modified with complementary biomolecules (e.g. antigen-antibody, biotin-avidin, ligand-receptor)
  • nanolithography, nanomechanical manipulations, ink-jet based deposition
  • AFM measurements, statistical analysis and data filtration, mechanical, electric and magnetic properties of samples
  • production, bioconjugation and deposition of nanoparticles (gold, magnetic, fluorescent QDs and other core/shell structures, upconverting, ...)