Structural Biology using Single Particle Cryo Electron Microscopy (SP CryoEM) has become a major tool for studying macromolecular assemblies. Visualising unprecedented details of cellular components, and solving in-situ protein structures are now possible with advancement in CryoEM methods. Developments in Field Emission Gun (FEG) Transmission Electron Microscope (TEM) and other TEM hardware, CryoEM specimen preparation methods, automated data acquisition systems, Direct Electron Detectors and improved image processing softwares have led to ~ 2000 proteins/protein-DNA/RNA complex structures larger than 52 kDa in size being routinely solved with < 4 Å resolution. The highest resolution obtained so far for a single particle EM map deposited in Electron Microscopy Data Bank (EMBD) is 1.54 Å.
However these cutting edge CryoEM methods for structural biology are relatively new to many labs in India and EMBC member states. There is an intense interest in the Indian and EMBC scientific community, particularly among structural biologists, about applications of this technique, which makes this course timely.
This EMBO Practical Course will deal with Single particle Cryo Electron Microscopy of biological macromolecular assemblies and Cryo Electron Tomography of Cellular structures. In addition, allied topics like MicroED is also introduced.
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