Key equipment: High-end electron microscope for high-resolution cryoEM and cryo-tomography FEI Titan Krios (80 - 300 kV) equipped with an energy filter and a direct detector camera. Conventional cryoEM microscope FEI F20 (200 kV) equipped with a CCD camera and a dual beam FIB/SEM instrument (Versa3D) for micromachining of thin lamellas of vitrified cells usable for electron tomography.
The cryo-EM core facility provides access and support to collect cryo-electron microscopy images for both single particle as well as for electron tomography applications. Furthermore, the facility provides support for sample preparation, namely plunge freezing of purified protein complexes as well as cryo-FIB lamella milling for thick biological objects such as cells. Additional support is given in experiment design, setting up data collection and data analysis (i.e. image processing). Interested non-specialists could receive training in using the electron microscopes or develop a collaboration with the CryoEM research group, particularly in these areas: