Mammalian Expression - Oxford

This facility is dedicated to protein expression in mammalian cells for structural biology. It includes (i) streamlined methodologies for transient expression in 293T cells, first for rapid small scale screening of multiple constructs, and then for scale up of selected constructs (Aricescu et al, 2006 Acta Cryst D), (ii) technologies for controlling glycosylation in secreted proteins (Chang et al, 2007, Structure) and SeMet labelling (e.g. Aricescu et al, 2006, EMBO J) and (iii) co-expression to produce heterodimeric protein complexes for structure determination. Development work includes membrane protein expression in mammalian cells.

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